CST, 67895S, SARS-CoV-2 Nsp7 Antibody

Polyclonal Antibody for studying nsp7-pp1a-pp1ab SARS-CoV-2/nsp7-pp1a-pp1ab SARS-CoV. Validated for Western Blotting. Highly specific and rigorously validated in-house, SARS-CoV-2 Nsp7 Antibody (CST #67895) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SARS-CoV-2 Nsp7 Antibody recognizes transfected levels of total SARS-CoV-2 Nsp7 protein. Species Reactivity: Virus Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala80 of SARS-CoV-2 Nsp7 protein. Antibodies are purified by peptide affinity chromatography. Background The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). Nsp7 (non-structural protein 7) is derived from ORF1a and ORF1b (2). It functions along with Nsp8, to bind to and cooperate with Nsp12 (RNA-dependent RNA polymerase) to greatly enhance the holoenzyme polymerase activity (3). Alternate Names non-structural protein 7; Non-structural protein 7; non-structural protein 7 (3860-3942); nsp7; nsp7-pp1a-pp1ab; nsp7-pp1a/pp1ab; SARS-CoV nsp7; SARS-CoV-2 nsp7 Specification REACTIVITY: Vir SENSITIVITY: Transfected Only MW (kDa): 10 SOURCE: Rabbit