CST, 68036T, PGM1 (E2I5G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PGM1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PGM1 (E2I5G) Rabbit Monoclonal Antibody (CST #68036) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PGM1 (E2I5G) Rabbit Monoclonal Antibody recognizes endogenous levels of total PGM1 protein. This antibody does not cross-react with PGM2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly279 of human PGM1 protein. Background Phosphoglucomutase-1 (PGM1) catalyzes the interconversion of glucose 1-phosphate (G-1-P) and glucose 6-phosphate (G-6-P) in the breakdown and synthesis of glycogen. PGM1 expression is downregulated in hepatocellular carcinoma (HCC), suppressing the glycogen synthesis pathway and increasing glucose entry into glycolysis to support cancer proliferation (1). Studies show that TGF-β1 released by ovarian cancer cells induces p38 MAPK signaling in neighboring cancer-associated fibroblasts (CAFs), releasing cytokines and chemokines from CAFs. These cytokines and chemokines promote glycogen mobilization in ovarian cancer cells, stimulating cancer cell proliferation, invasion, and metastasis. Phosphorylation of Tyr353 on PGM1 is essential for its enzymatic activity and glycogen mobilization induced by CAFs (2). Alternate Names CDG1T; Glucose phosphomutase 1; GSD14; PGM 1; PGM1; phosphoglucomutase 1; Phosphoglucomutase-1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG