CST, 68179S, Cyclin C (E6V4Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Cyclin C. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Cyclin C (E6V4Z) Rabbit Monoclonal Antibody (CST #68179) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:6400 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 73204 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Cyclin C (E6V4Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total cyclin C protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro263 of human cyclin C protein. Background Cyclin dependent kinases (CDKs) are regulated in part through binding to regulatory cyclins. While many cyclin/CDK complexes regulate progression through the cell cycle, others, such as cyclin C/CDK8, regulate transcription and other cellular functions. Cyclin C/CDK8 forms a regulatory sub-complex with Med12 and Med13, which associates with the multi-subunit transcriptional coactivator Mediator Complex to modulate RNA polymerase II-dependent transcription. In addition to its role in transcription, cyclin C is important in the regulation of mitochondrial dynamics, apoptosis, and cell cycle progression (1). Cytosolic cyclin C induces mitochondrial fragmentation and apoptosis, and is required for activation of the Bcl2 family member, Bax (2). Cytoplasmic cyclin C also supports mitochondrial fission through dynamin-related protein 1 (Drp1) in response to oxidative stress (3). Cyclin C acts synergistically with PTEN to maintain levels of tumor suppressors p53 and p21 in a mouse model of thyroid cancer (4). In complex with CDK3, cyclin C regulates Rb-dependent exit from quiescence in human cells (5). Alternate Names CCNC; CycC; cyclin C; Cyclin-C; hSRB11; SRB11; SRB11 homolog Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG