CST, 68191S, TR4/NR2C2 Antibody

Polyclonal Antibody for studying NR2C2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, TR4/NR2C2 Antibody (CST #68191) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TR4/NR2C2 Antibody recognizes endogenous levels of total TR4/NR2C2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile10 of human TR4/NR2C2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Testicular receptor 4 (TR4), also called TAK1 or NR2C2, is an orphan receptor in a distinct subclass of the steroid hormone nuclear receptor superfamily along with TR2 (1,2). TR4 forms heterodimers with TR2 and binds to DNA elements containing direct repeats (DRs) (3). TR4 plays a role in various processes, including neurogenesis, spermatogenesis, RNA metabolism, and protein translation (4-6). TR4 can interact with other hormone receptors such as AR and ER to influence protein-protein binding and target gene inactivation (7,8). High expression of TR4 correlates with prostate cancer metastasis and invasion through downstream targets such as CCL2 and Ezh2 (9-10). Expression of TR4 in other cancers such as NSCLC and testicular germ cell tumors has also been associated with poor prognosis (11-12). Alternate Names hTAK1; NR2C2; Nuclear hormone receptor TR4; Nuclear receptor subfamily 2 group C member 2; nuclear receptor subfamily 2, group C, member 2; Orphan nuclear receptor TAK1; Orphan nuclear receptor TR4; orphan receptor TR4; TAK1; testicular nuclear receptor 4; Testicular receptor 4; TR2R1; TR4; TR4 nuclear hormone receptor Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 67 SOURCE: Rabbit