CST, 68236SF, Phospho-PDGF Receptor alpha (Tyr849)/PDGF Receptor beta (Tyr857) (C43E9) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying PDGFR-a (Tyr849) phosphate/PDGFR-b (Tyr857) phosphate. Validated for Western Blotting,ELISA+. Highly specific and rigorously validated in-house, Phospho-PDGF Receptor alpha (Tyr849)/PDGF Receptor beta (Tyr857) (C43E9) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #68236) is ready to ship. Product Usage Information This product is the carrier free version of product #3170. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 3170 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Phospho-PDGF Receptor alpha (Tyr849)/PDGF Receptor beta (Tyr857) (C43E9) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of PDGF receptor α and β only when phosphorylated on Tyr849 of PDGFRα and Tyr857 of PDGFRβ. This antibody may cross-react with other activated tyrosine kinases. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr857 of human PDGF receptor β. Background Platelet derived growth factor (PDGF) family proteins exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ). PDGFRα and PDGFRβ share 75% to 85% sequence homology between their two intracellular kinase domains, while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGFRα homodimers bind all PDGF isoforms except those containing PDGF D. PDGFRβ homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF receptor α/β binds PDGF B, C, and D homodimers, as well as the PDGF AB heterodimer (2). PDGFRα and PDGFRβ can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLCγ, and NCK. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation (5). Tyr751 in the kinase-insert region of PDGFRβ is the docking site for PI3 kinase (6). Phosphorylated pentapeptides derived from Tyr751 of PDGFRβ (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRβ (7). Tyr740 is also required for PDGFRβ-mediated PI3 kinase activation (8). Alternate Names Activated tyrosine kinase PDGFRB; Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140; CD140 antigen-like family member A; CD140 antigen-like family member B; CD140A; CD140a antigen; CD140B; IBGC4; IMF1; JTK12; KOGS; MGC74795; NDEL1-PDGFRB; PDGF-R-alpha; PDGF-R-beta; PDGFR; PDGFR alpha; PDGFR beta; PDGFR-1; PDGFR-2; PDGFR-alpha; PDGFR-beta; PDGFR1; PDGFR2; PDGFRA; PDGFRalpha; PDGFRB; PENTT; PGFRA; PGFRB; platelet derived growth factor receptor alpha; platelet derived growth factor receptor beta; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 1; Platelet-derived growth factor receptor 2; Platelet-derived growth factor receptor alpha; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor, alpha polypeptide; platelet-derived growth factor receptor, beta polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alpha fusion protein; RHEPDGFRA Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 190 Source/Isotype: Rabbit IgG