CST, 68280S, DGCR8 (E9O3L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DGCR8 mouse. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, DGCR8 (E9O3L) Rabbit Monoclonal Antibody (CST #68280) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:200 Chromatin IP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity DGCR8 (E9O3L) Rabbit Monoclonal Antibody recognizes endogenous levels of total DGCR8 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro767 of mouse DGCR8 protein. Background Drosha was identified as a nuclear RNase III that catalyzes the initial step of microRNA (miRNA) processing (1). This enzyme processes the long primary transcript pri-miRNAs into stem-looped pre-miRNAs. Interference of Drosha results in the increase of pri-miRNAs and the decrease of pre-miRNAs (1). Drosha exists in a multiprotein complex called Microprocessor along with other components, such as DGCR8 (2). Drosha and DGCR8 are necessary for miRNA biogenesis (3). Alternate Names D16H22S1742E; D16H22S788; D16H22S788E; D16Wi; D16Wis2; Dgcr8; DGCR8, microprocessor complex subunit; DiGeorge syndrome critical region 8 homolog; DiGeorge syndrome critical region gene 8; Gy1; Microprocessor complex subunit DGCR8; mir-1306; N41; OTTMUSP00000027994; OTTMUSP00000027995; Vo59c; Vo59c07 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 120 Source/Isotype: Rabbit IgG