CST, 68351S, CD177 (E6G1H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD177. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CD177 (E6G1H) Rabbit Monoclonal Antibody (CST #68351) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CD177 (E6G1H) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD177 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CD177 protein. Background Cluster of differentiation 177 antigen (CD177, also known as human neutrophil alloantigen 2a and NB1), is a glycosyl-phosphatidylinositol (GPI)-linked N-glycosylated transmembrane protein expressed primarily on the surface of neutrophils (1,2). Expression of CD177 can be induced by granulocyte-colony-stimulating factor and during pregnancy or bacterial infection (3-5). CD177 mediates adhesion and transendothelial migration of neutrophils via interaction with CD31/PECAM-1 and integrin β2 on endothelial cells (6,7). CD177 also promotes the cell surface expression of proteinase 3 (PR3), which may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (8-10). In association with CD11b/ITGAM, integrin β2, and PR3, CD177 promotes activation of TNF-α primed neutrophils including degranulation and superoxide production (11).  is a polymorphic gene that has been linked to several important clinical diseases, including polycythemia vera, Wegener's granulomatosis, and immune mediated neonatal neutropenia (12-14). Alternate Names CD177; CD177 antigen; CD177 molecule; cell surface receptor; HNA-2a; HNA2A; Human neutrophil alloantigen 2a; NB1; NB1 glycoprotein; NB1 GP; polycythemia rubra vera 1; Polycythemia rubra vera protein 1; PRV-1; PRV1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 48, 65 Source/Isotype: Rabbit IgG