CST, 68390S, CD24 (M1/69) Rat Monoclonal Antibody (APC Conjugate)

Monoclonal Antibody for studying CD24 mouse. Validated for Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD24 (M1/69) Rat Monoclonal Antibody (APC Conjugate) (CST #68390) is ready to ship. Product Usage Information For optimal flow cytometry results, we recommend 0.06 μg of antibody per test. Flow Cytometry (Fixed/Permeabilized): 1:330 Flow Cytometry (Live): 1:330 Storage Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity CD24 (M1/69) Rat Monoclonal Antibody (APC Conjugate) recognizes endogenous levels of total CD24 protein. This antibody conjugate detects an epitope within the extracellular domain. Species Reactivity: Mouse Source / Purification This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Background CD24, also know as heat stable antigen HSA, is a P-selectin ligand involved in adhesion. It is a GPI-anchored glycoprotein expressed on many types of cells, including hematopoietic cells, neural cells, and epithelial cells. CD24 is widely used to delineate stages of lymphocyte development (1-3). It also binds to Siglec-10 in humans or Siglec-G in mice (4,5). CD24 is frequently used as a marker to identify and isolate cancer stem cells in various cancer types (6,7). Alternate Names CD24; Cd24a; CD24a antigen; cluster of differentiation 24; heat stable antigen; HSA; Ly-5; Ly-52; lymphocyte antigen 52; M1/69-J11D heat stable antigen; nect; nectadrin; R13-Ag; Signal transducer CD24; X62 heat stable antigen Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rat IgG2b kappa