CST, 68522T, CIRBP (D1M8D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CIRBP. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CIRBP (D1M8D) Rabbit Monoclonal Antibody (CST #68522) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CIRBP (D1M8D) Rabbit Monoclonal Antibody recognizes endogenous levels of total CIRBP protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly150 of human CIRBP protein. Background Cold-induced RNA-binding protein (CIRBP) is a 172-residue, multifunctional sensor protein that was first isolated as a protein induced in mouse fibroblasts cultured at 32ºC (1). Conversely, CIRBP expression decreases in cells or tissues subjected to increased temperature (2). The CIRBP protein is composed of an amino-terminal RNA-binding domain and a carboxyl-terminal, glycine-rich domain (1). Stressful stimuli, such as hypoxia, heat shock, osmotic shock, or oxidative conditions, lead to translocation of CIRBP from the nucleus to cytoplasmic stress granules through a mechanism involving CIRBP methylation-dependent nuclear export (3). CIRBP plays a role in regulating apoptosis and preserving the stemness of neural stem cells at moderately low temperatures (4). Research studies demonstrate that CIRBP contributes to the regulation of circadian rhythm through post-translational modulation of CLOCK expression (5). Alternate Names A18 hnRNP; A18HNRNP; CIRBP; CIRP; cold inducible RNA binding protein; cold inducible RNA-binding protein; Cold-inducible RNA-binding protein; glycine-rich RNA binding protein; Glycine-rich RNA-binding protein CIRP; testicular tissue protein Li 39 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG