CST, 68533T, PAPSS1 (E6U5C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PAPSS1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PAPSS1 (E6U5C) Rabbit Monoclonal Antibody (CST #68533) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PAPSS1 (E6U5C) Rabbit Monoclonal Antibody recognizes endogenous levels of total PAPSS1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PAPSS1 protein. Background PAPS Synthase 1 and 2 (PAPSS1, PAPSS2) are bifunctional enzymes with both ATP sulfurylase and APS kinase activity. They mediate two reactions to generate 3'-phosphoadenosine-5'-phosphosulfate (PAPS), a critical intermediate in the sulfate activation pathway. PAPS is the sulfate donor co-substrate for all sulfotransferase (SULT) enzymes and is required to catalyze the sulfate conjugation of many endogenous and exogenous compounds (1,2). For example, PAPSS1 and PAPSS2 are responsible for catalyzing the sulfation of steroid hormones, which increases their water solubility and reduces their biological activity, suggesting modulation of PAPS synthases as a possible avenue for treatment of hormone-dependent cancers (3). Inhibition of PAPSS1 was also shown to increase the sensitivity of some cancer cell lines to DNA-damage agents both and (4,5), suggesting further potential utility for PAPS synthase inhibitors in cancer therapy. Alternate Names 3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 1; 3'-phosphoadenosine 5'-phosphosulfate synthase 1; 3'-phosphoadenosine-5'-phosphosulfate synthase; Adenosine-5'-phosphosulfate 3'-phosphotransferase; Adenylyl-sulfate kinase; Adenylylsulfate 3'-phosphotransferase; APS kinase; ATP-sulfurylase; ATPSK1; Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1; PAPS synthase 1; PAPS1; PAPSS; PAPSS 1; PAPSS1; SAT; SK 1; SK1; Sulfate adenylate transferase; Sulfate adenylyltransferase; Sulfurylase kinase 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG