CST, 68534S, Occludin (6B8A3) Rat Monoclonal Antibody

Monoclonal Antibody for studying occludin. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Occludin (6B8A3) Rat Monoclonal Antibody (CST #68534) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Occludin (6B8A3) Rat Monoclonal Antibody recognizes endogenous levels of total occludin protein. In some human tissue lysates, this antibody detects a band of unknown origin at approximately 95 kDa by western blot. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of mouse occludin protein. Background Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). Tight junctions are composed of claudin and occludin transmembrane proteins, which join the junctions to the cytoskeleton (1,2). Occludin is thought to be important in the assembly and maintenance of tight junctions. Differential phosphorylation of occludin at various residues may regulate its interaction with other tight junction proteins such as ZO-1 (3). VEGF-induced phosphorylation of occludin regulates tight junction stability and vascular permeability (4). Expression of occludin as well as claudin1 is required for infection of liver cells by hepatitis C virus (HCV) (5). Alternate Names BLCPMG; hCG_1988850; Occludin; OCLN; phosphatase 1, regulatory subunit 115; PPP1R115; PTORCH1; tight junction protein occludin TM4 minus Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 65 Source/Isotype: Rat IgG2a