CST, 68540S, PGK1 Antibody

Polyclonal Antibody for studying PGK1. Validated for Western Blotting. Highly specific and rigorously validated in-house, PGK1 Antibody (CST #68540) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PGK1 Antibody recognizes endogenous levels of total PGK1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr298 of human PGK1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background PGK1 (phosphoglycerate kinase) is an essential enzyme in the glycolysis pathway (1). It catalyzes the reversible phospho-transfer reaction from 1,3-diphosphoglycerate to ADP to form ATP and 3-phosphoglycerate. The expression of PGK1 is upregulated in many cancer types and plays an important role in cancer cell proliferation and metastasis (2-5). PGK1 can also function as a protein kinase. ERK can phosphorylate PGK1 at Ser203. This phosphorylation changes PGK1 conformation and leads to its translocation from cytoplasm to mitochondria. There it interacts and phosphorylates PDHK1 (pyruvate dehydrogenase kinase isomerase 1), which leads to PDHK1 inhibition, blocks pyruvate to coenzyme A conversion, and promotes cytosolic lactate concentration (6). Acetylated PGK1 can phosphorylate Beclin1 to induce autophagy (7). PGK1 is activated by autophosphorylation at Tyr324. PTEN can dephosphorylate PGK1 at this site to downregulate its activity. In cancer cells, loss of PTEN enhances PGK1 activity and promotes glycolysis and tumor growth (8). Alternate Names Cell migration-inducing gene 10 protein; epididymis secretory sperm binding protein Li 68p; HEL-S-68p; MGC117307; MGC142128; MGC8947; MIG10; PGK1; PGKA; Phosphoglycerate kinase 1; Primer recognition protein 2; PRP 2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 43 SOURCE: Rabbit