CST, 68591S, Lamin B1 (119D5-F1) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Lamin B1. Validated for Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Lamin B1 (119D5-F1) Mouse Monoclonal Antibody (CST #68591) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied at 1 mg/mL in PBS containing 0.09% sodium azide. Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present, but will not interfere with antibody performance. This product is stable for 36 months when stored at -20C. Protocol Available protocols: Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Lamin B1 (119D5-F1) Mouse Monoclonal Antibody recognizes endogenous levels of total Lamin B1 by immunofluroescence. Testing by immunofluorescence was validated for human cells. Reactivity is expected in other species, including mouse and rat. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with purified rat liver lamins. Background Lamins are nuclear membrane structural components that are important in maintaining normal cell functions, such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamins have been subdivided into types A and B. Type-A lamins consist of lamin A and C, which arise from alternative splicing of the lamin A gene . Lamin A and C are cleaved by caspases into large (41-50 kDa) and small (28 kDa) fragments, which can be used as markers for apoptosis (4,5). Type-B lamins consist of lamin B1 and B2, encoded by separate genes (6-8). Lamin B1 is also cleaved by caspases during apoptosis (9). Research studies have shown that duplication of the lamin B1 gene is correlated with pathogenesis of the neurological disorder adult-onset leukodystrophy (10). Alternate Names ADLD; lamin B1; Lamin-B1; LMN; LMN2; LMNB; LMNB1; MGC111419 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1