CST, 68711SF, BATF3 (E3F3L) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying BATF3. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, BATF3 (E3F3L) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #68711) is ready to ship. Product Usage Information This product is the carrier free version of product #72565. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 72565 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity BATF3 (E3F3L) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total BATF3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human BATF3 protein. Background BATF3 (Basic leucine zipper transcriptional factor ATF-like 3, p21SNFT) is a member of the basic leucine zipper family (1,2,5) which dimerizes with Jun proteins and represses activator protein-1 (AP-1) activity (1). BATF3 is required for development of conventional type 1 dendritic cells (cDC1s), which cross-present antigens in the context of MHC class I to naïve CD8 T cells, priming them to become activated cytotoxic CD8 T cells (2-4). Alternate Names 21 kDa small nuclear factor isolated from T-cells; 21-kD small nuclear factor isolated from T cells; B-ATF-3; basic leucine zipper ATF-like transcription factor 3; basic leucine zipper transcription factor, ATF-like 3; Basic leucine zipper transcriptional factor ATF-like 3; BATF3; FLJ36352; FLJ37535; JDP1; Jun dimerization protein 1; Jun dimerization protein p21SNFT; JUNDM1; SNFT Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG