CST, 68796S, Methionine Synthase (D3D6H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MTR. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Methionine Synthase (D3D6H) Rabbit Monoclonal Antibody (CST #68796) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Methionine Synthase (D3D6H) Rabbit Monoclonal Antibody recognizes endogenous levels of total methionine synthase protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1178 of human methionine synthase protein. Background One-carbon metabolism includes enzymatic reactions involving the transfer of one-carbon groups mediated by folate cofactor. The activated one-carbon groups are used by various metabolic pathways, including purine synthesis, thymidine synthesis, and remethylation of homocysteine to methionine (1). One of the enzymes in one-carbon metabolism, methionine synthase, catalyzes the conversion of 5-methyltetrahydrofolate and homocysteine to tetrahydrofolate and methionine. Methionine is further converted to S-adenosylmethionine (SAM) (1, 2). S-adenosylmethionine (SAM) is a major reactive methyl carrier and plays a critical role in epigenetic regulation (2, 3). Alternate Names 5-methyltetrahydrofolate--homocysteine methyltransferase; 5-methyltetrahydrofolate-homocysteine methyltransferase; 5-methyltetrahydrofolate-homocysteine methyltransferase 1; cblG; cobalamin-dependent methionine synthase; FLJ33168; FLJ43216; FLJ45386; HMAG; METH; Methionine synthase; MS; MTR; Vitamin-B12 dependent methionine synthase Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Rabbit IgG