CST, 68797SF, Pdx1 (D59H3) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying PDX1. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Pdx1 (D59H3) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #68797) is ready to ship. Product Usage Information This product is the carrier free version of product #5679. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 5679 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Pdx1 (D59H3) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total Pdx1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val274 of human Pdx1 protein. Background The pancreatic duodenal homeobox gene-1 (Pdx1) is a transcription factor that contributes to pancreas development, pancreatic β-cell differentiation, and mature β-cell function (1,2). It plays an essential role in the commitment of endoderm to a pancreatic and later β-cell phenotype (2,3). In the mature pancreas, Pdx1 expression is more restricted to the pancreatic β-cells (3), where it promotes the expression of genes important for β-cell functions such as insulin, glucokinase, and Glut2 (4-6). Mutations of the corresponding Pdx1 gene may be associated with diabetes and cases of pancreatic insufficiency (7). Alternate Names Glucose-sensitive factor; GSF; IDX-1; Insulin promoter factor 1; insulin promoter factor 1, homeodomain transcription factor; Insulin upstream factor 1; IPF-1; IPF1; Islet/duodenum homeobox-1; IUF-1; IUF1; MODY4; PAGEN1; Pancreas/duodenum homeobox protein 1; pancreatic and duodenal homeobox 1; pancreatic-duodenal homeobox factor 1; PDX-1; PDX1; somatostatin transcription factor 1; Somatostatin-transactivating factor 1; STF-1; STF1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG