CST, 68836T, beta-Dystroglycan (E3Z8H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Dystroglycan. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, beta-Dystroglycan (E3Z8H) Rabbit Monoclonal Antibody (CST #68836) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity beta-Dystroglycan (E3Z8H) Rabbit Monoclonal Antibody recognizes endogenous levels of total β-dystroglycan protein. This antibody does not cross-react with α-dystroglycan protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro690 of human dystroglycan 1 protein. Background Dystroglycan 1 (DAG1) is a ubiquitously expressed glycoprotein that is post-translationally processed into two protein subunits. α-dystroglycan is secreted and localizes to the outer plasma membrane, where it binds to the transmembrane subunit, β-dystroglycan. The two proteins form a complex that connects the cytoskeleton with the extracellular matrix and plays essential roles in various cellular processes, including neuromuscular junction function and adhesion-driven cell signaling (1,2). Phosphorylation of β-dystroglycan at Tyr892 regulates protein-protein interaction and subcellular localization (3-5). β-dystroglycan interacts with nuclear envelope proteins emerin and lamin B1 to regulate nuclear architecture and function (6). Alternate Names 156DAG; A3a; AGRNR; Alpha-DG; Alpha-dystroglycan; Beta-DG; Beta-dystroglycan; DAG; DAG1; Dystroglycan; Dystroglycan 1; dystroglycan 1 (dystrophin-associated glycoprotein 1); Dystrophin-associated glycoprotein 1; LGMDR16; MDDGA9; MDDGC7; MDDGC9 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG