CST, 68862T, SF3B4 (E7R4H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SF3B4. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SF3B4 (E7R4H) Rabbit Monoclonal Antibody (CST #68862) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SF3B4 (E7R4H) Rabbit Monoclonal Antibody recognizes endogenous levels of total SF3B4 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro207 of human SF3B4 protein. Background SF3B4 is part of the SF3b complex, which associates with the U2 snRNP component of the spliceosome and is essential for spliceosome assembly (1-3). SF3B4 can associate with BMPR-1A to inhibit BMP-mediated cell differentiation (4). Haploinsufficiency of SF3B4 has been described in Nager syndrome, a disease of craniofacial malformation condition (5,6). Increased copy number of SF3B4 has been shown to play a role in hepatocellular carcinoma progression (7-9). Alternate Names AFD1; Hsh49; MGC10828; pre-mRNA splicing factor SF3b 49 kDa subunit; Pre-mRNA-splicing factor SF3b 49 kDa subunit; SAP 49; SAP49; SF3B4; SF3b49; SF3b50; spliceosomal protein; spliceosome-associated protein (U2 snRNP); Spliceosome-associated protein 49; Splicing factor 3B subunit 4; splicing factor 3b, subunit 4, 49kD; splicing factor 3b, subunit 4, 49kDa Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG