CST, 6892S, SMC1 (8E6) Mouse Monoclonal Antibody

Monoclonal Antibody for studying SMC1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SMC1 (8E6) Mouse Monoclonal Antibody (CST #6892) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SMC1 (8E6) Mouse Monoclonal Antibody recognizes endogenous levels of total SMC1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human SMC1 protein. Background The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2, or 3) (1,2). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms; however, cohesin remains bound to centromeres until anaphase (3,4). RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis (5). RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64 kDa carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis (6,7). In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage (1,2). Alternate Names CDLS2; DKFZp686L19178; DXS423E; EIEE85; epididymis secretory sperm binding protein; KIAA0178; MGC138332; SB1.8; segregation of mitotic chromosomes 1; SMC protein 1A; SMC-1-alpha; SMC-1A; SMC1; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC1 structural maintenance of chromosomes 1-like 1; SMC1A; SMC1alpha; SMC1L1; SMCB; structural maintenance of chromosomes 1A; Structural maintenance of chromosomes protein 1A Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 145 Source/Isotype: Mouse IgG1