CST, 68978S, MAGE-A10 Antibody

Polyclonal Antibody for studying MAGE-A10. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, MAGE-A10 Antibody (CST #68978) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MAGE-A10 Antibody recognizes endogenous levels of total MAGE-A10 protein. This antibody does not cross-react with other MAGE-A family members. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro323 of human MAGE-A10 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Cancer/testis antigens (CTAs) are a family of more than 100 proteins whose normal expression is largely restricted to immune privileged germ cells of the testis, ovary, and trophoblast cells of the placenta. Although most normal somatic tissues are void of CTA expression, due to epigenetic silencing of gene expression, their expression is upregulated in a wide variety of human solid and liquid tumors (1,2). As such, CTAs have garnered much attention as attractive targets for a variety of immunotherapy-based approaches to selectively attack tumors (3). Melanoma-associated antigen-A10 (MAGE-A10) is a cancer testis antigen and belongs to the type I MAGE family of proteins. Unlike other members of the MAGE-A subfamily, MAGE-A10 is a nuclear protein (4). Research studies have shown that MAGE-A10 expression is normally restricted to the human testis and placenta but is aberrantly upregulated in a number of human solid tumors, such as lung cancer, melanoma, bladder cancer, and esophageal carcinoma (4-6). Due to its upregulated expression in human tumors and high degree of immunogenicity, MAGE-A10 has received significant attention as a novel immunotherapy target through the use of vaccines and adoptive cell therapy (7-9). Alternate Names Cancer/testis antigen 1.10; cancer/testis antigen family 1, member 10; CT1.10; MAGAA; MAGE family member A10; MAGE-10 antigen; MAGE-A10; MAGE10; MAGEA10; melanoma antigen family A, 10; melanoma antigen family A10; Melanoma-associated antigen 10; MGC10599 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 62 SOURCE: Rabbit