CST, 6897T, Bassoon (D63B6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BSN. Validated for Western Blotting,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Bassoon (D63B6) Rabbit Monoclonal Antibody (CST #6897) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 69024 . Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen) Specificity / Sensitivity Bassoon (D63B6) Rabbit Monoclonal Antibody recognizes endogenous levels of total Bassoon protein. Some background staining was observed in muscle tissue and the choroid plexus by confocal immunofluorescent analysis. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln1217 of human bassoon protein. Background Bassoon (BSN), named such to underline its presumptive role in orchestrating events of the synaptic vesicle cycle (1), is a very large coiled-coil protein and is one of the core components of the cytomatrix at the active zones of both excitatory and inhibitory synapses (2). BSN is a scaffold protein that is a component of the synaptic ribbon, an electron-dense structure anchored at the active zone that tethers synaptic vesicles. Genetic disruption of BSN displaces the anchoring of ribbons to the active zones of photoreceptors and cochlear inner hair cells (3), and this displacement of the ribbons substantially impairs synaptic transmission, suggesting that, when present, BSN is important for the vesicle cycle (4). Alternate Names bassoon (presynaptic cytomatrix protein); bassoon presynaptic cytomatrix protein; BSN; KIAA0434; neuronal double zinc finger protein; Protein bassoon; Zinc finger protein 231; ZNF231 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 420 Source/Isotype: Rabbit IgG