CST, 69024SF, Bassoon (D63B6) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying BSN. Validated for Western Blotting,Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, Bassoon (D63B6) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #69024) is ready to ship. Product Usage Information This product is the carrier free version of product #6897. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 6897 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Bassoon (D63B6) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Bassoon protein. Some background staining was observed in muscle tissue and the choroid plexus by confocal immunofluorescent analysis. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln1217 of human bassoon protein. Background Bassoon (BSN), named such to underline its presumptive role in orchestrating events of the synaptic vesicle cycle (1), is a very large coiled-coil protein and is one of the core components of the cytomatrix at the active zones of both excitatory and inhibitory synapses (2). BSN is a scaffold protein that is a component of the synaptic ribbon, an electron-dense structure anchored at the active zone that tethers synaptic vesicles. Genetic disruption of BSN displaces the anchoring of ribbons to the active zones of photoreceptors and cochlear inner hair cells (3), and this displacement of the ribbons substantially impairs synaptic transmission, suggesting that, when present, BSN is important for the vesicle cycle (4). Alternate Names bassoon (presynaptic cytomatrix protein); bassoon presynaptic cytomatrix protein; BSN; KIAA0434; neuronal double zinc finger protein; Protein bassoon; Zinc finger protein 231; ZNF231 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 420 Source/Isotype: Rabbit IgG