CST, 6915S, Miwi (D92B7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Miwi mouse. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Miwi (D92B7) Rabbit Monoclonal Antibody (CST #6915) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:800 - 1:3200 Immunofluorescence (Frozen): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity Miwi (D92B7) Rabbit Monoclonal Antibody recognizes endogenous levels of total Miwi protein. Non-specific staining was observed in a subset of mouse immune cells by immunohistochemistry. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly81 of mouse Miwi protein. Background The piwi gene was identified as being required for the self-renewal of germline stem cells (1). Piwi homologs are well conserved among various species including , and (1). Both Miwi and Mili proteins are mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mice (3-5). Alternate Names MGC150073; Miwi; piwi like homolog 1; piwi-like homolog 1; piwi-like homolog 1 (Drosophila); Piwi-like protein 1; piwi-like RNA-mediated gene silencing 1; Piwil1; PIWL1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG