CST, 6919S, SIK2 (D28G3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SIK2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SIK2 (D28G3) Rabbit Monoclonal Antibody (CST #6919) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SIK2 (D28G3) Rabbit Monoclonal Antibody recognizes endogenous levels of total SIK2 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro14 of human SIK2 protein. Background Salt-inducible kinase 1 (SIK1) was originally identified as a serine/threonine kinase from adrenocortical tissues of rats on a high salt diet (1). SIK1 is an SNF1/AMPK family kinase capable of autophosphorylation (1). SIK2 is an isoform of SIK1 and is specifically expressed in adipose tissues where it is induced during adipocyte differentiation (2). Studies suggest that SIK2 can phosphorylate human insulin receptor substrate 1 (IRS-1) at Ser794. Along with evidence that SIK2 expression and activity are increased in white adipocytes of diabetic mice, this finding suggests a possible role for SIK2 in regulating insulin signaling in adipocytes and in the development of insulin resistance (2,3). Insulin triggers Akt2-mediated phosphorylation of SIK2 at Ser358 and the resultant kinase activation during post-fasting feeding (4). The activated SIK2 then induces the phosphorylation of TORC2 at Ser171, resulting in translocation of this transcriptional coactivator from the nucleus to the cytoplasm, where it is degraded through the ubiquitin pathway, leading to inhibition of gluconeogenic gene expression (4). Alternate Names DKFZp434K1115; KIAA0781; LOH11CR1I; QIK; Qin-induced kinase; salt inducible kinase 2; salt-inducible kinase 2; Salt-inducible protein kinase 2; salt-inducible serine/threonine kinase 2; Serine/threonine-protein kinase SIK2; Serine/threonine-protein kinase SNF1-like kinase 2; SIK-2; SIK2; SNF1-like kinase 2; SNF1LK2 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG