CST, 69204S, SIX3 Antibody

Polyclonal Antibody for studying SIX3. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, SIX3 Antibody (CST #69204) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SIX3 Antibody recognizes endogenous levels of total SIX3 protein. This antibody is not predicted to cross-react with other SIX proteins, based on sequence divergence in the epitope region. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg281 of human SIX3 protein. Antibodies are purified by peptide affinity chromatography. Background Sine oculis homeobox (SIX) proteins belong to a family of evolutionarily conserved transcription factors discovered in mutant screens for embryonic eye development genes (1-3). The prototypical family member ( , ) was named for eyeless embryos carrying mutations in a gene highly conserved among vertebrates, including humans ( ) (4). A total of six family members (SIX1-6) have been identified in vertebrates. Each SIX protein contains a homeobox nucleic acid recognition domain (HD) with a DNA-binding helix-turn-helix motif and an adjacent SIX domain, which may be involved in regulating protein-protein interactions (5). In addition to their critical functions during embryonic organogenesis, research studies suggest that SIX proteins play additional roles in postnatal cell cycle regulation, with potentially important implications in tumorigenesis (6,7). In addition to its well-established role within gene networks regulating neural patterning and brain development (8,9), SIX3 was more recently reported to regulate postnatal pancreatic β cell maturation, in part by suppressing the expression of genes associate with fetal β cells and various non-β cell types (10,11). Dysregulated expression of SIX3 has also been linked with tumor development. For example, in a study of cell free DNA isolated from plasma, was identified as a differentially methylated gene that was diagnostically relevant for patients with pancreatic ductal adenocarcinoma (12). This finding was consistent with earlier reports supporting a role for SIX3 as a tumor suppressor (13-15). Alternate Names Homeobox protein SIX3; HPE2; Sine oculis homeobox homolog 3; sine oculis homeobox-like protein 3; SIX homeobox 3; SIX3; truncated SIX3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30, 38, 45 SOURCE: Rabbit