CST, 6924S, PCK2 Antibody

Polyclonal Antibody for studying PCK2. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, PCK2 Antibody (CST #6924) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity PCK2 Antibody recognizes endogenous levels of total PCK2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PCK2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background PCK2 (PEPCK2 or PEPCK-M) encodes an isoform of phosphoenolpyruvate carboxykinase (PEPCK) that is found in the mitochondria of renal and hepatic tissues (1). PEPCK is involved in gluconeogenesis, the process of generating glucose from non-carbohydrate substrates such as lactate and glycerol (2). PEPCK catalyzes the reaction whereby oxaloacetate becomes phosphoenolpyruvate, a rate limiting step in the metabolic process (2). The predominant function of PCK2 is to process the lactate that is continually produced by red blood cells in the liver and kidney (1). Alternate Names epididymis secretory sperm binding protein; PCK2; PCKGM; PEP carboxykinase; PEPCK; PEPCK-M; PEPCK2; Phosphoenolpyruvate carboxykinase [GTP], mitochondrial; phosphoenolpyruvate carboxykinase 2 (mitochondrial); phosphoenolpyruvate carboxykinase 2, mitochondrial; Phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 71 SOURCE: Rabbit