CST, 6931T, CCT alpha (D18B6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PCYT1A. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CCT alpha (D18B6) Rabbit Monoclonal Antibody (CST #6931) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CCT alpha (D18B6) Rabbit Monoclonal Antibody recognizes endogenous levels of total CCTα protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CCTα protein. Background CTP:phosphocholine cytidylyltransferase (CCT) is a critical enzyme that regulates the CDP-choline pathway for the biosynthesis of phosphatidylcholine. Three distinct CCT isoforms are found in mammals, including CCTα, CCTβ2, and CCTβ3 (1,2). CCTα is the major isoform that is expressed in most tissues (3). CCTα is essential in the synthesis and secretion of surfactant by alveolar epithelial cells and is important in maintaining the phosphatidylcholine level that regulates lipoprotein assembly and secretion in hepatocytes (4,5). CCTα is a major component in membrane biogenesis during cytokine secretion by stimulated macrophages (6). Monoubiquitination of CCTα prevents it from entering the nucleus and leads to its degradation by lysosome (7). Alternate Names CCT A; CCT-alpha; CCTA; Choline-phosphate cytidylyltransferase A; CT; CT A; CTA; CTP:phosphocholine cytidylyltransferase A; CTPCT; PCY1A; PCYT1; PCYT1A; phosphate cytidylyltransferase 1, choline, alpha; Phosphorylcholine transferase A; SMDCRD Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG