CST, 69324SF, TNF-alpha (D1G2) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying TNF-alpha. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, TNF-alpha (D1G2) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #69324) is ready to ship. Product Usage Information This product is the carrier free version of product #8184. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8184 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity TNF-alpha (D1G2) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total TNF-α protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human TNF-α protein. Background TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk1/2, p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably (3). Alternate Names APC1 protein; C-domain 1; C-domain 2; Cachectin; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; TNF; TNF superfamily, member 2; TNF-a; TNF-alpha; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNFSF2; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 18, 25 Source/Isotype: Rabbit IgG