CST, 69363S, Phospho-Paxillin (Tyr118) (E9U9F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Paxillin (Tyr118) phosphate. Validated for WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Paxillin (Tyr118) (E9U9F) Rabbit Monoclonal Antibody (CST #69363) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:200 - 1:400 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 91115 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Phospho-Paxillin (Tyr118) (E9U9F) Rabbit Monoclonal Antibody recognizes endogenous levels of paxillin protein only when phosphorylated at Tyr118. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr118 of human paxillin protein. Background Paxillin is a multidomain protein that localizes primarily to focal adhesion sites in the extracellular matrix (1). Paxillin is one of the key components of integrin signaling, and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by another focal adhesion component, focal adhesion kinase (FAK), at Tyr118 (3,4). Phospho-paxillin (Tyr118) may provide a docking site for recruitment of other signaling molecules to focal adhesions. It has been shown that the SH2 domain of Crk binds to the phosphorylated Tyr118 of paxillin (5). Alternate Names FLJ16691; PAXI; Paxillin; PXN; testicular tissue protein Li 134 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 68 Source/Isotype: Rabbit IgG