CST, 69375T, NFI-A (E6Z1R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NFI-A. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, NFI-A (E6Z1R) Rabbit Monoclonal Antibody (CST #69375) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Chromatin IP Specificity / Sensitivity NFI-A (E6Z1R) Rabbit Monoclonal Antibody recognizes endogenous levels of total NFI-A protein. Based on sequence homology, this antibody is not predicted to cross-react with other NFI family members. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro430 of human NFI-A protein. Background NFI-A belongs to the nuclear factor I (NFI) family of site-specific transcription factors that regulate viral DNA replication and expression of various genes (1,2). The NFI family is composed of four members in vertebrates: NFI-A, NFI-B, NFI-C, and NFI-X, all of which are critical in the development of multiple organ systems in mice and humans (3). NFI-A activates cell-type-specific enhancers and facilitates the binding of PPARγ, the master transcriptional regulator of adipogenesis, to control the brown fat gene program (4). Additionally, NFI-A represses the expression of , the master transcriptional regulator of myogenesis, thus actively suppressing the myogenic gene program (5). Alternate Names BRMUTD; CCAAT-box-binding transcription factor; CTF; DKFZp434L0422; DKFZp686J23256; FLJ39164; KIAA1439; NF-I/A; NF1-A; NFI-A; NFI-L; NFIA; Nuclear factor 1 A-type; Nuclear factor 1/A; nuclear factor I A; Nuclear factor I/A; TGGCA-binding protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG