CST, 69387S, MAP4K3 (E9L1J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MAP4K3 mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MAP4K3 (E9L1J) Rabbit Monoclonal Antibody (CST #69387) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MAP4K3 (E9L1J) Rabbit Monoclonal Antibody recognizes endogenous levels of total MAP4K3 protein. A band of unknown identity is detected at 48 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His404 of human MAP4K3 protein. Background The mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by growth factors and nutrients including amino acids (1,2). MAP4K3 is a mediator between nutrient signal and mTORC1 (1). Research studies suggest that amino acid sufficiency leads to the phosphorylation of Ser170 on MAP4K3, which activates mTORC1 (3). Alternate Names 4833416M01Rik; 4833416M07Rik; 9530052P13Rik; germinal center kinase-like kinase-like; Germinal center kinase-related protein kinase; GLK; KHS2; M4K3; Map4k3; MAPK/ERK kinase kinase kinase 3; MAPKKKK3; MEK kinase kinase 3; MEKKK 3; MEKKK3; mitogen activated protein kinase kinase kinase kinase 3; Mitogen-activated protein kinase kinase kinase kinase 3; RAB8IPL1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 101 Source/Isotype: Rabbit IgG