CST, 69399S, Dynactin p150Glued (D1W1O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying dynactin 1. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Dynactin p150Glued (D1W1O) Rabbit Monoclonal Antibody (CST #69399) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity Dynactin p150Glued (D1W1O) Rabbit Monoclonal Antibody recognizes endogenous levels of total dynactin p150Glued protein. Based on the amino acid sequence of the peptide antigen used, this antibody is expected to detect all isoforms of dynactin p150Glued, including p135. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1206 of human dynactin p150Glued protein. Background Cytoplasmic dynein is a multi-subunit motor complex that regulates microtubule organization as well as the transport and positioning of organelles. Dynactin is a multi-subunit dynein-activating complex, which regulates the interaction of the dynein motor with various cellular cargoes, and enhances dynein's processivity. p150Glued/DCTN1/Dynactin 1 is the largest subunit of the dynactin complex (1-3). In mitosis, cytoplasmic dynein regulates spindle organization, chromosome movement and centrosome separation (4). The dynactin subunit p150Glued is phosphorylated at serine 19 by the mitotic kinase aurora A during anaphase, and this phosphorylation is required for the appropriate regulation of spindle assembly (5). In neurons, axonal transport is important for cellular function and survival. Dysfunction and mutations in dynein and dynactin subunits, including p150Glued, have been linked to human neurodegenerative diseases such as Alzheimer's Disease (6-7), Perry Syndrome (8) and ALS (9). Alternate Names 150 kDa dynein-associated polypeptide; DAP-150; DCTN1; DP-150; dynactin 1; dynactin 1 (p150, glued homolog, Drosophila); Dynactin subunit 1; HMN7B; p135; p150-glued; p150, Glued homolog Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 150 Source/Isotype: Rabbit IgG