CST, 6945T, TNF-alpha (D5G9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TNF-alpha. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TNF-alpha (D5G9) Rabbit Monoclonal Antibody (CST #6945) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TNF-alpha (D5G9) Rabbit Monoclonal Antibody recognizes endogenous levels of total full length and secreted TNF-α protein. This antibody can detect up to 10 pg of recombinant TNF-α by western blot. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human TNF-α protein. Background TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk1/2, p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably (3). Alternate Names APC1 protein; C-domain 1; C-domain 2; Cachectin; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; TNF; TNF superfamily, member 2; TNF-a; TNF-alpha; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNFSF2; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 18, 25 Source/Isotype: Rabbit IgG