CST, 69542T, DNAJC9 (F4U7W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DNAJC9. Validated for Western Blotting,Simple Western™,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, DNAJC9 (F4U7W) Rabbit Monoclonal Antibody (CST #69542) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity DNAJC9 (F4U7W) Rabbit mAb recognizes endogenous levels of total DNAJC9 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala236 of human DNAJC9 protein. Background DnaJ homolog subfamily C member 9 (DNAJC9) serves a dual role as both a histone chaperone and a heat shock co-chaperone. As a histone chaperone, DNAJC9 forms a co-chaperone complex with MCM2 and histone H3-H4 heterodimers, potentially assisting MCM2 in recognizing these heterodimers and facilitating their assembly into nucleosomes. DNAJC9 interacts with HSP70, activating its ATPase activity (1). Overexpression of CENP-A, a centromeric histone H3 variant, is frequently observed in cancer. Its mislocalization to noncentromeric regions promotes chromosomal instability (CIN), a hallmark of cancer. DNAJC9 plays a crucial role in preventing the mislocalization of centromeric CENP-A, thereby mitigating chromosome instability (2). DNAJC9 expression is a potential prognostic biomarker in basal-like and luminal A breast cancer (3). Alternate Names DnaJ (Hsp40) homolog, subfamily C, member 9; DnaJ heat shock protein family (Hsp40) member C9; DnaJ homolog subfamily C member 9; DnaJ protein SB73; DNAJC9; DNJC9; HDJC9; JDD1; KIAA0974; SB73 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG