CST, 69569S, ANT1/SLC25A4 Antibody

Polyclonal Antibody for studying SLC25A4/ANT1. Validated for Western Blotting. Highly specific and rigorously validated in-house, ANT1/SLC25A4 Antibody (CST #69569) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ANT1/SLC25A4 Antibody recognizes endogenous levels of total ANT1/SLC25A4 protein. This antibody does not cross-react with ANT2/SLC25A5 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala150 of human ANT1/SLC25A4 protein. Antibodies are purified by peptide affinity chromatography. Background Adenine nucleotide translocators (ANTs) are a family of mitochondrial proteins that exchange ATP and ADP across the mitochondrial inner membrane. ANT1, also known as solute carrier family 25 member 4 (SLC25A4), is the isoform predominantly expressed in heart, muscle, and brain (1,2). Homozygous loss-of-function mutations in causes cardiomyopathy (2,3). In addition, ANT1/SLC25A4 is shown to be required for mitophagy (3). Furthermore, research studies show that ANT1/SLC25A4 facilitates mitochondrial permeability transition induced by bile acids and, therefore, pyroptosis (4). ANT1/SLC25A4 may mediate the release of ATP from mitochondria to the cytoplasm in this process, which is critical for pyroptosome assembly (4). Alternate Names AAC1; Adenine nucleotide translocator 1; adenine nucleotide translocator 1 (skeletal muscle); adenine nucleotide translocator), member 4; ADP,ATP carrier protein 1; ADP,ATP carrier protein, heart/skeletal muscle; ADP,ATP carrier protein, heart/skeletal muscle isoform T1; ADP/ATP translocase 1; ADT1; ANT; ANT 1; ANT1; heart/skeletal muscle ATP/ADP translocator; MTDPS12; MTDPS12A; PEO2; PEO3; PEOA2; SLC25A4; solute carrier family 25 (mitochondrial carrier; Solute carrier family 25 member 4; T1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 28 SOURCE: Rabbit