CST, 69589S, Adavosertib

Chemical Modulators for studying in the research area. Product Usage Information Adavosertib is supplied as a lyophilized powder. For a 10 mM stock, reconstitute 5 mg of powder in 999 μL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect. Storage Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles. Background Adavosertib (MK-1775) is a potent, selective Wee1 kinase inhibitor with an IC value of 5.2 nM. Adavosertib inhibits phosphorylation of the Wee1 substrate Cdc2 at Tyr15, thereby abrogating the G2 DNA damage checkpoint (1). Wee1 inhibition in p53-deficient tumors by Adavosertib has been shown to inhibit and regress tumor growth (2). Alternate Names 1,2-dihydro-1-[6-(1-hydroxy-1-methylethyl)-2-pyridinyl]-6-[[4-(4-methyl-1- piperazinyl)phenyl]amino]-2-(2-propen-1-yl)-3H-pyrazolo[3,4-d]pyrimidin-3-one; AZD 1775; MK-1775