CST, 69659SF, Evi-1 (C50E12) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying EVI1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Evi-1 (C50E12) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #69659) is ready to ship. Product Usage Information This product is the carrier free version of product #2593. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 2593 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Evi-1 (C50E12) Rabbit Monoclonal Antibody (BSA and Azide Free) detects the endogenous levels of total Evi-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp57 of human Evi-1. Background Evi-1 (Ecotropic virus integration site 1) was originally identified as a common site of viral integration in murine myeloid leukemia. It is involved in human myeloid disorders through chromosome translocation and inversion (1) and is also implicated in solid tumor formation (2). Evi-1 is a zinc finger transcription factor which also plays an important role in animal development (3). It has many isoforms due to alternative usage of 5'-ends (4), alternative splicing (5), and intergenic splicing which results in the formation of a fusion protein with MDS1 in normal tissues (6). Alternate Names AML1-EVI-1; AML1-EVI-1 fusion partner; AML1-EVI-1 fusion protein; ecotropic viral integration site 1; Ecotropic virus integration site 1 protein homolog; EVI-1; EVI1; Histone-lysine N-methyltransferase MECOM; KMT8E; MDS1; MDS1 and EVI1 complex locus; MDS1 and EVI1 complex locus protein; MDS1 and EVI1 complex locus protein EVI1; MDS1 and EVI1 complex locus protein MDS1; MDS1-EVI1; MECOM; MGC163392; MGC97004; myelodysplasia syndrome 1 protein; myelodysplasia syndrome-associated protein 1; oncogene EVI1; PR domain 3; PRDM3; RUSAT2; zinc finger protein Evi1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 105, 145, 200 Source/Isotype: Rabbit IgG