CST, 69661S, Sox10 (E6B6I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SOX10. Validated for WB,IP,IHC,IHC. Available in 2 sizes. Highly specific and rigorously validated in-house, Sox10 (E6B6I) Rabbit Monoclonal Antibody (CST #69661) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 IHC Leica Bond: 1:50 - 1:200 Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . For a carrier free (BSA and azide free) version of this product see product # 88386 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity Sox10 (E6B6I) Rabbit Monoclonal Antibody recognizes endogenous levels of total Sox10 protein. This antibody also recognizes presumptive sumoylated forms of Sox10 protein. Non-specific staining was observed in neuroendocrine cells of the stomach by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly446 of human Sox10 protein. Background SRY-box 10 (Sox10) is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and the determination of cell fate. Sox10 is an important regulator of neural crest and peripheral nervous system development (1-3). Mutations in the gene are associated with a group of auditory-pigmentary developmental disorders, including Waardenburg and Waardenburg-Shah syndromes (3,4). Research studies suggest an oncogenic role for Sox10 in various tumor types, such as hepatocellular carcinoma and melanoma (5,6). Alternate Names DOM; dominant megacolon, mouse, human homolog of; MGC15649; PCWH; SOX10; SRY (sex determining region Y)-box 10; SRY-box 10; SRY-box transcription factor 10; SRY-related HMG-box gene 10; Transcription factor SOX-10; WS2E; WS4; WS4C Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG