CST, 69683S, PHB1 (E8R3V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PHB. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PHB1 (E8R3V) Rabbit Monoclonal Antibody (CST #69683) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PHB1 (E8R3V) Rabbit Monoclonal Antibody recognizes endogenous levels of total PHB1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human PHB1 protein. Background The prohibitins PHB1 and PHB2 are highly conserved, multifunctional proteins present in eukaryotic nuclear and mitochondrial compartments. PHB1 is a 30 kDa tumor suppressor protein involved in cell cycle control (1). PHB1 has been found in mitochondria, the nucleus, and the plasma membrane, as well as extracellularly in circulation (2). In mitochondria, prohibitins mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation (3,4). In the nucleus, prohibitins interact with transcription factors such as Rb and p53 to regulate target gene transcription (2,5). Extracellular prohibitin can bind and activate C3 to enhance complement activation (6). Alternate Names epididymis luminal protein 215; epididymis secretory sperm binding protein Li 54e; HEL-215; HEL-S-54e; PHB; PHB1; Prohibitin; Prohibitin 1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 29 Source/Isotype: Rabbit IgG