CST, 69734SF, CGRP (D5R8F) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CGRP. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, CGRP (D5R8F) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #69734) is ready to ship. Product Usage Information This product is the carrier free version of product #14959. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 14959 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CGRP (D5R8F) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CGRP protein. Rat cross-reactivity is determined by Immunofluorescence. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val114 of human CGRP protein. Background Calcitonin gene-related peptide (CGRP) is a peptide of 37 amino acids that belongs to the calcitonin (CT) family of peptide hormones. The calcitonin gene ( ) encodes a number of tissue-specific peptides through alternative splicing of mRNA transcripts and precursor protein cleavage (1). Both calcitonin and α-CGRP are produced from the CALCA gene, while a second gene ( ) encodes the related β-CGRP protein (2). α-CGRP and β-CGRP share similar activities and differ by three or fewer residues depending on the species (3). The CGRP peptide activates a heterotrimeric receptor complex that consists of the seven transmembrane-spanning calcitonin receptor-like receptor, the single transmembrane-spanning RAMP1 protein, and an intracellular receptor component protein (4,5). CGRP is expressed in the central and peripheral nervous system in mammals, where it exhibits several important physiologic roles. Research studies demonstrate that CGRP is a potent vasodilatator (6) and a modulator of acetylcholine receptor function at neuromuscular junctions (7). Additional studies indicate that CGRP peptide is involved in feeding (8) and inflammatory pain (9). CGRP peptide also plays a key role in the physiology of migraine attacks. Specifically, CGRP peptide levels increase during acute migraine attacks, which can be ameliorated through treatment with CGRP antagonists (10). Alternate Names Alpha-type CGRP; CALC1; CALCA; CALCA iso3; calcitonin; calcitonin 1; Calcitonin gene-related peptide 1; Calcitonin gene-related peptide I; calcitonin related polypeptide alpha; calcitonin-related polypeptide alpha; calcitonin/calcitonin-related polypeptide, alpha; CGRP; CGRP-alpha; CGRP-I; CGRP1; CT; katacalcin; KC; MGC126648; PCT Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 5 Source/Isotype: Rabbit IgG