CST, 69758S, alpha-Actinin (E7U1O) Mouse Monoclonal Antibody

Monoclonal Antibody for studying ACTN1. Validated for WB,WB,IP,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, alpha-Actinin (E7U1O) Mouse Monoclonal Antibody (CST #69758) is ready to ship. Product Usage Information Western Blotting: 1:1000 Fluorescent Western: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Fluorescent Western, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity alpha-Actinin (E7U1O) Mouse Monoclonal Antibody recognizes endogenous levels of total α-Actinin protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human α-Actinin-1 protein. Background α-Actinin belongs to the spectrin family of cytoskeletal proteins. It was first recognized as an actin cross-linking protein, forming an antiparallel homodimer with an actin binding head at the amino terminus of each monomer. The α-actinin protein interacts with a large number of proteins involved in signaling to the cytoskeleton, including those involved in cellular adhesion, migration, and immune cell targeting (1). The interaction of α-actinin with intercellular adhesion molecule-5 (ICAM-5) helps to promote neurite outgrowth (2). In osteoblasts, interaction of α-actinin with integrins stabilizes focal adhesions and may protect cells from apoptosis (3). The cytoskeletal α-actinin isoforms 1 and 4 (ACTN1, ACTN4) are non-muscle proteins that are present in stress fibers, sites of adhesion and intercellular contacts, filopodia, and lamellipodia. The muscle isoforms 2 and 3 (ACTN2, ACTN3) localize to the Z-discs of striated muscle and to dense bodies and plaques in smooth muscle (1). Alternate Names actinin 1 smooth muscle; actinin alpha 1; actinin, alpha 1; ACTN1; alpha-actinin; alpha-actinin 1; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; BDPLT15; epididymis secretory sperm binding protein; F-actin cross-linking protein; FLJ40884; FLJ54432; Non-muscle alpha-actinin-1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Mouse IgG1