CST, 69766S, P2Y12 (E9J1J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying P2Y12 mouse. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, P2Y12 (E9J1J) Rabbit Monoclonal Antibody (CST #69766) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:1600 - 1:6400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 38216 . Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity P2Y12 (E9J1J) Rabbit Monoclonal Antibody recognizes endogenous levels of total P2Y12 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro173 of mouse P2Y12 protein. Background Purinergic signaling involves cell signaling pathways that are activated by nucleosides and nucleotides via specific purinergic receptors (1). Purinergic receptors include two large families, P1 receptors that are activated by nucleosides and P2 receptors that are activated by nucleotides. P2 receptors are further divided into ionotropic P2X receptors and metabotropic P2Y receptors. P2Y purinoceptor 12 (P2Y12) is a member of the P2Y receptor family (2). As G protein-coupled receptors, P2Y12 are activated by ADP and ATP to drive downstream second messenger pathways. P2Y12 is required for normal platelet aggregation and blood coagulation (3). In addition to their role in thrombosis, P2Y12 receptors drive purinergic signaling in the central nervous system (CNS). P2Y12 receptors are enriched in microglia and regulate nucleotide-dependent microglia chemotaxis and morphology (4). Furthermore, P2Y12 receptors regulate specialized microglia/neuron junctions that provide microglia-dependent protection of neuronal functions in response to brain injury and disease (5). Alternate Names 2900079b22rik; 4921504d23rik; p2ry12; P2Y purinoceptor 12; P2Y1; p2y12; purinergic receptor p2y, g-protein coupled 12; purinergic receptor p2y12 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG