CST, 6976S, PAR2 (D61D5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PAR-2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PAR2 (D61D5) Rabbit Monoclonal Antibody (CST #6976) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PAR2 (D61D5) Rabbit Monoclonal Antibody recognizes endogenous levels of total PAR2 protein. The major band detected runs at 32 kDa, the higher molecular weight smear is due to glycosylation. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly44 of human PAR2 protein. Background PAR2 (F2RL1) belongs to the PAR (Protease-activated Receptor) family of G protein-coupled receptors. These membrane receptors are activated through N-terminal cleavage of the receptor by a serine protease such as thrombin, trypsin, or matrix metalloproteinases (1,2). This cleavage exposes the ‘tethered-ligand' fragment of the receptor, which binds to a second extracellular loop of the receptor and leads to receptor activation. PAR2 is specifically activated by trypsin or trypsin-like proteases. Activated PAR2 stimulates phosphoinositide hydrolysis and calcium mobilization, interacts with β-arrestin, and eventually leads to ERK activation (3). PAR2 expression and activation are mainly associated with inflammatory diseases (3), but may also play a role in cancer development (4,5). Alternate Names coagulation factor II (thrombin) receptor-like 1; Coagulation factor II receptor-like 1; F2R like trypsin receptor 1; F2RL1; G protein-coupled receptor-11; G-protein coupled receptor 11; GPR11; PAR-2; PAR2; protease-activated receptor 2; Proteinase-activated receptor 2; Proteinase-activated receptor 2, alternate cleaved 1; Proteinase-activated receptor 2, alternate cleaved 2; proteinase-activated receptor-2; Thrombin receptor-like 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 32 Source/Isotype: Rabbit IgG