CST, 69817S, GM-CSF Receptor alpha (E8Z3S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CSF2RA. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, GM-CSF Receptor alpha (E8Z3S) Rabbit Monoclonal Antibody (CST #69817) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:200 - 1:800 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63107 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity GM-CSF Receptor alpha (E8Z3S) Rabbit Monoclonal Antibody recognizes endogenous levels of total GM-CSF Receptor α protein. This antibody does not cross-react with GM-CSF Receptor β protein. Based on the antigen sequence, this antibody is predicted to recognize multiple isoforms of GM-CSF Receptor α protein, including secreted isoforms. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human GM-CSF Receptor α protein. Background Granulocyte-macrophage colony-stimulating factor receptor subunit alpha (GM-CSF Receptor α), also known as CSF2RA and CD116, is a type 1 transmembrane protein with low affinity for GM-CSF. GM-CSF Receptor α is primarily expressed on myeloid cells, including granulocytes, monocytes, macrophages, and dendritic cells (1). GM-CSF Receptor α forms a heterodimeric receptor complex with GM-CSF Receptor β, creating the high affinity GM-CSF receptor (2). Upon dimerization and ligand binding, the β subunit becomes phosphorylated and transduces signals via Jak/STAT and MAPK pathways for cell proliferation, differentiation, and survival (3,4). Oligomerization of the high affinity GM-CSF Receptor α/β complex is required for optimal intracellular signal transduction (5). Soluble GM-CSF Receptor α, either secreted or cleaved from the cell surface, can competitively bind GM-CSF, preventing membrane receptor signaling (1,6,7). Multiple isoforms of GM-CSF Receptor α have been described. Alternate Names alpha-GM-CSF receptor; alphaGMR; CD116; CD116 antigen; CDw116; colony stimulating factor 2 receptor alpha subunit; colony stimulating factor 2 receptor subunit alpha; colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage); CSF2R; CSF2RA; CSF2RAX; CSF2RAY; CSF2RX; CSF2RY; GM-CSF receptor alpha subunit; GM-CSF-R-alpha; GMCSFR; GMCSFR-alpha; GMR; GMR-alpha; granulocyte-macrophage colony-stimulating factor receptor alpha chain; Granulocyte-macrophage colony-stimulating factor receptor subunit alpha; SMDP4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70-80 Source/Isotype: Rabbit IgG