CST, 6981S, Phospho-Rad17 (Ser645) (D5H5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Rad17 (Ser656) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Rad17 (Ser645) (D5H5) Rabbit Monoclonal Antibody (CST #6981) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-Rad17 (Ser645) (D5H5) Rabbit Monoclonal Antibody recognizes endogenous levels of Rad17 protein only when phosphorylated at Ser645. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser645 of human Rad17 protein. Background The human checkpoint protein Rad17 and its fission and budding yeast orthologues ( Rad17 and Rad24, respectively) are involved in the activation of checkpoint signals in response to DNA damage or disruption of DNA synthesis (1-4). Treatment of human cells with genotoxic agents induces ATM/ATR-dependent phosphorylation of Rad17 at Ser635 and Ser645. Rad17 phosphorylation is a critical early event during checkpoint signaling in DNA-damaged cells (5-7). Alternate Names CCYC; cell cycle checkpoint protein (RAD17); Cell cycle checkpoint protein RAD17; FLJ41520; hRad17; R24L; RAD1 homolog; RAD17; RAD17 checkpoint clamp loader component; RAD17 homolog; RAD17 homolog (S. pombe); Rad17-like protein; RAD17SP; RAD24; RF-C activator 1 homolog; RF-C/activator 1 homolog Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG