CST, 69882T, RBM25 (F9X4K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RBM25. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, RBM25 (F9X4K) Rabbit Monoclonal Antibody (CST #69882) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity RBM25 (F9X4K) Rabbit Monoclonal Antibody recognizes endogenous levels of total RBM25 protein. This antibody detects a 150 kDa band of unknown origin. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro225 of human RBM25 protein. Background RBM25 is an RNA-binding protein involved in alternative splicing of a large number of transcripts (1,2). RBM25 contributes to splicing by binding splicing factor SRSF2, a process that is blocked by the methylation of RBM25 on Lys77 (2). In various cancer contexts, RBM25 can act as a tumor suppressor. It is capable of selecting for a proapoptotic variant of BCL-x, and can control the Mac inhibitor BIN1 (1,3). In other cancer types, such as hepatocellular carcinoma (HCC), high RBM25 expression has been shown to be associated with poor prognosis (4). RBM25 is also involved in cardiac processes by abnormally splicing SCN5A, truncating it to a form associated with sudden cardiac failure (5,6). In Alzheimer's disease (AD), RBM25 can form aggregates with the U1 small nuclear ribonucleoprotein 70 kDa (U1-70K) and localize to neurofibrillary Tau aggregates (7). Alternate Names Arg/Glu/Asp-rich protein of 120 kDa; Arg/Glu/Asp-rich protein, 120 kDa; fSAP94; functional spliceosome-associated protein 94; MGC105088; MGC117168; NET52; Protein S164; RBM25; RED120; RNA binding motif protein 25; RNA-binding motif protein 25; RNA-binding protein 25; RNA-binding region (RNP1, RRM) containing 7; RNA-binding region-containing protein 7; RNPC7; S164; Snu71; U1 small nuclear ribonucleoprotein 1SNRP homolog Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 120 Source/Isotype: Rabbit IgG