CST, 69908T, EAAT4 (E6E9I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SLC1A6. Validated for Western Blotting,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, EAAT4 (E6E9I) Rabbit Monoclonal Antibody (CST #69908) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:200 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen) Specificity / Sensitivity EAAT4 (E6E9I) Rabbit Monoclonal Antibody recognizes endogenous levels of total EAAT4 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly552 of human EAAT4 protein. Background During neurotransmission, glutamate is released from vesicles of the presynaptic cell, and glutamate receptors (e.g., NMDA receptor, AMPA receptor) bind glutamate for activation at the opposing postsynaptic cell. Excitatory amino acid transporters (EAATs) regulate and maintain extracellular glutamate concentrations below excitotoxic levels (1,2). In addition, glutamate transporters may limit the duration of synaptic excitation by an electrogenic process in which the transmitter is cotransported with three sodium ions and one proton, followed by countertransport of a potassium ion (1,2). Five EAATs (EAAT1-5) have been identified. EAAT1 and EAAT2 are expressed mainly in glia, while EAAT3, EAAT4, and EAAT5 are considered to be neuronal transporters (2). EAAT4 (encoded by the gene) is a neuronal glutamate transporter expressed in Purkinje cells of the cerebellum (3). EAAT4 plays an important role in maintaining neuronal synaptic function, particularly in the cerebellum, by maintaining appropriate extracellular glutamate levels to prevent neurotoxicity and permit effective synaptic communication. EAAT4 function may be regulated by post-translational modification (4,5). EAAT4 may be a therapeutic target to treat several neurological diseases, including spinocerebellar ataxias, by restoring extracellular glutamate homeostasis (6). Alternate Names EAA4; EAAT4; Excitatory amino acid transporter 4; MGC33092; MGC43671; SLC1A6; Sodium-dependent glutamate/aspartate transporter; solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6; Solute carrier family 1 member 6 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 20, 22, 50, 70, 80 Source/Isotype: Rabbit IgG