CST, 69926S, MMP-13 (E4W3T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MMP13. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MMP-13 (E4W3T) Rabbit Monoclonal Antibody (CST #69926) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MMP-13 (E4W3T) Rabbit Monoclonal Antibody recognizes endogenous levels of total human MMP13 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human MMP13 protein. Background MMP-13 (collagenase 3) belongs to the matrix metalloproteinase (MMP) superfamily of enzymes that targets many extracellular proteins, including other proteases, growth factors, cell surface receptors, and adhesion molecules (1,2). MMP-13 is a member of a subgroup of collagenases (including MMP-1, MMP-8, and MMP-18) that play an even more important function targeting fibrillar collagen. MMP-13 is synthesized as a latent proenzyme, and proteolytic removal of the inhibitory propeptide domain is required for enzyme activation. MMP-13 protein levels are regulated at the transcriptional level via specific transcription factors and promoter DNA methylation (3,4). MMP-13 preferentially cleaves Type II collagen, and research studies have shown that aberrant upregulation of MMP-13 activity can lead to cartilage loss and osteoarthritis (5,6). In addition, MMP-13 has been shown to promote cancer development, in part through enhancing tumor angiogenesis and metastases (7-9), suggesting that collagenase activity may serve as a useful marker of tumor progression (10). Alternate Names CLG3; Collagenase 3; MANDP1; matrix metallopeptidase 13; matrix metallopeptidase 13 (collagenase 3); matrix metalloproteinase 13; matrix metalloproteinase 13 (collagenase 3); Matrix metalloproteinase-13; MDST; MMP-13; MMP13 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG