CST, 69974SF, ASCT2 (D7C12) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying ASCT2. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, ASCT2 (D7C12) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #69974) is ready to ship. Product Usage Information This product is the carrier free version of product #8057. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8057 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity ASCT2 (D7C12) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total ASCT2 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ASCT2 protein. Background Sodium-dependent neutral amino acid transporter type 2 (ASCT2 or SLC1A5) is a neutral amino acid transporter that regulates the uptake of essential amino acids in conjunction with the SLC7A5 bilateral transporter (1,2). ASCT2 appears to be the major glutamine transporter in hepatoma cells and is thought to provide essential amino acids needed for tumor growth (3). Additional evidence suggests that ASCT2 plays a role in activating mTORC1 signaling and is required to suppress autophagy (4,5). Cell surface ASCT2 serves as a receptor for several mammalian interference retroviruses associated with cases of infectious immunodeficiency; variation in a small region of an extracellular loop (ECL2) may be responsible for species-specific differences in receptor function (6). Alternate Names AAAT; ASCT2; ATB(0); ATBO; Baboon M7 virus receptor; FLJ31068; M7V1; M7VS1; neutral amino acid transporter B; Neutral amino acid transporter B(0); R16; RD114 virus receptor; RD114/simian type D retrovirus receptor; RDR; RDRC; SLC1A5; Sodium-dependent neutral amino acid transporter type 2; solute carrier family 1 (neutral amino acid transporter), member 5; Solute carrier family 1 member 5 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 49, 75 Source/Isotype: Rabbit