CST, 70008T, c-IAP1 (E6R2S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying cIAP1 mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, c-IAP1 (E6R2S) Rabbit Monoclonal Antibody (CST #70008) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity c-IAP1 (E6R2S) Rabbit Monoclonal Antibody recognizes endogenous levels of total c-IAP1 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse c-IAP1 protein. Background The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase (2). Alternate Names Api1; Api2; apoptosis inhibitor 2; AW146227; baculoviral IAP repeat-containing 2; baculoviral IAP repeat-containing 3; Baculoviral IAP repeat-containing protein 2; Birc2; Birc3; C-IAP1; C330006D17Rik; Cellular inhibitor of apoptosis 1; cIA; cIAP1; cIAP2; HIAP1; HIAP2; IAP-2; IAP1; Iap2; Inhibitor of apoptosis protein 2; mcI; mcIAP1; mIAP-2; MIAP1; mIAP2; MIH; MIHB; MIHC; RING-type E3 ubiquitin transferase BIRC2; RNF48 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG